Chikungunya virus (CHIKV) E2 Protein, His Tag (MALS verified)

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E2P-V52H3-50ug
¥4725.00
E2P-V52H3-500ug
¥29400.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    E2

  • 表达区间及表达系统(Source)

    Chikungunya virus (CHIKV) E2 Protein, His Tag (E2P-V52H3) is expressed from human 293 cells (HEK293). It contains AA Ser 326 - Gln 666 (Accession # Q1H8W5).

    Predicted N-terminus: Ser 326

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    E2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 40.3 kDa. The protein migrates as 48-63 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    E2 SDS-PAGE

    Chikungunya virus (CHIKV) E2 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    E2 SEC-MALS

    The purity of Chikungunya virus (CHIKV) E2 Protein, His Tag (Cat. No. E2P-V52H3) is more than 85% and the molecular weight of this protein is around 38-58 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     E2 ELISA

    Immobilized Chikungunya virus (CHIKV) E2 Protein, His Tag (Cat. No. E2P-V52H3) at 1 μg/mL (100 μL/well) can bind CHIKV E2 Antibody (8E12) Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).

    Protocol

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背景介绍

The envelope protein 2 (E2) of Chikungunya virus (CHIKV) is a key surface glycoprotein responsible for host cell receptor binding and viral entry. It forms heterodimers with the E1 fusion protein and assembles into trimeric spikes on the viral surface. E2 contains major neutralizing epitopes, making it a prime target for vaccine development and serological diagnostics.

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