Human E-Cadherin / Cadherin-1 (155-709) Protein, Fc Tag (MALS verified)

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ECD-H5256-100ug
¥2865.00
ECD-H5256-1mg (250ug X 4)
¥16800.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    Cadherin-1,CDH1,CD324,E-cadherin,Arc-1,ECAD,LCAM,UVO

  • 表达区间及表达系统(Source)

    Human E-Cadherin (155-709) Protein, Fc Tag (ECD-H5256) is expressed from human 293 cells (HEK293). It contains AA Asp 155 - Ala 709 (Accession # P12830-1).

    Predicted N-terminus: Asp 155

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    E-Cadherin Structure

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 87.0 kDa . The protein migrates as 50 kDa and 95-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    E-Cadherin SDS-PAGE

    Human E-Cadherin (155-709) Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    E-Cadherin SEC-MALS

    The purity of Human E-Cadherin (155-709) Protein, Fc Tag (Cat. No. ECD-H5256) is more than 90% and the molecular weight of this protein is around 200-230 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     E-Cadherin ELISA

    Immobilized Mouse KLRG1, His Tag (Cat. No. KL1-M5249) at 5 μg/mL (100 μL/well) can bind Human E-Cadherin (155-709) Protein, Fc Tag (Cat. No. ECD-H5256) with a linear range of 0.02-1.25 μg/mL (QC tested).

    Protocol

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背景介绍

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Cadherin-1 (CDH1) is also known as epithelial cadherin (E-cadherin), CD_antigen (CD324), Uvomorulin (UVO) ECAD and CDHE, CDH1 / CD324 contains 5 cadherin domains. CDH1 / CD324 / ECAD is expressed in non-neural epithelial tissues. CDH1 / E-CAD is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells and has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad promotes non-amyloidogenic degradation of Abeta precursors and has a strong inhibitory effect on APP C99 and C83 production. Defects in CDH1 / CD324 / ECAD are the cause of hereditary diffuse gastric cancer (HDGC).

前沿进展

 
药物研发进展
  • 英文全称:

    Cadherin-1

  • 中文全称:

    钙粘蛋白-1

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    0 详情

  • 最高研发阶段:

    临床前

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