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分子别名（Synonym）
Aromatic-L-amino-acid decarboxylase, Dopa decarboxilase, AADC
表达区间及表达系统（Source）
Human DDC, His Tag (DDC-H55H6) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Glu 480 (Accession # P20711-1).
Predicted N-terminus: Met 1
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 55.8 kDa. The protein migrates as 53-58 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 25 mM MES, 150 mM NaCl, pH6.0 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Human DDC, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human DDC, His Tag (Cat. No. DDC-H55H6) is more than 95% and the molecular weight of this protein is around 100-125 kDa verified by SEC-MALS.
Report
活性（Bioactivity）
Measured by its ability to convert the substrate 3, 4dihydroxy Lphenylalanine (LDopa) to 3, 4dihydroxyphenylethylamine (dopamine). The dopamine product is measured by its absorbance at 340 nm after derivatization with trinitrobenzene sulfonic acid. The specific activity is >1000 pmol/min/µg (QC tested).
Protocol
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