Human CYB5R3 Protein, His Tag

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CY3-H5143-100ug
¥3675.00
CY3-H5143-1mg
¥24255.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    CYB5R3

  • 表达区间及表达系统(Source)

    Human CYB5R3 Protein, His Tag (CY3-H5143) is expressed from E. coli cells. It contains AA Lys 2 - Phe 278 (Accession # P00387-2).

    Predicted N-terminus: Met

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    CYB5R3 Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 33.5 kDa. The protein migrates as 33-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    CYB5R3 SDS-PAGE

    Human CYB5R3 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

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背景介绍

CYB5R3 (Cytochrome B5 Reductase 3) encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias.

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