Human CXCR2 Protein, Flag,His Tag (Detergent)

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CX2-H55D3-20ug
¥7350.00
CX2-H55D3-100ug
¥27300.00
CX2-H55D3-500ug
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产品详情

  • 分子别名(Synonym)

    CD182, CDw128b, CMKAR2, IL8R2, IL8RA, IL8RB, CXCR2, CXC-R2, CXCR-2, GRO, MGSA receptor, IL-8R B

  • 表达区间及表达系统(Source)

    Human CXCR2 Protein, Flag,His Tag (CX2-H55D3) is expressed from Baculovirus-Insect cells. It contains AA Glu 2 - Ser 344 (Accession # P25025).

    Predicted N-terminus: Asp

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    CXCR2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a flag tag at the N-terminus and a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 43.0 kDa.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    This product is not suitable for cell based experiments due to cytotoxicity of DDM.
    DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumstances should you remove DDM and CHS.
    DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
    If glycerol is not compatible with your application, remove glycerol just before the immediate experiment, and NEVER store glycerol-free protein solution.

    Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, pH7.5, CHS with glycerol as protectant.

    Contact us for customized product form or formulation.

  • 运输(Shipping)

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储(Storage)

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程
  • *The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow this link for product information.

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数据展示

  • 活性(Bioactivity)-ELISA

     CXCR2 ELISA

    Immobilized Human CXCR2 Protein, Flag,His Tag (Cat. No. CX2-H55D3) at 1 μg/mL (100 μL/well) on a Nickel Coated plate (Cat. No. SP-19) can bind Anti-CXCR2 Antibody with a linear range of 0.2-3 ng/mL (QC tested).

    Protocol
  • 活性(Bioactivity)-SPR

     CXCR2 SPR

    Monoclonal Anti-CXCR2 Antibody, Human IgG4 captured on Protein A Chip can bind Human CXCR2 Protein, Flag,His Tag (Cat. No. CX2-H55D3) with an affinity constant of 10.8 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).

    Protocol

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背景介绍

The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009]

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