Human Complement Factor B Protein, His Tag (MALS & SPR verified)

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CFB-H52H3-100ug
¥3500.00
CFB-H52H3-1mg
¥23100.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    AHUS4, ARMD14, BF, BFD, CFAB, CFBD, FB, FBI12, GBG, H2-Bf, PBF2

  • 表达区间及表达系统(Source)

    Human Complement Factor B Protein, His Tag (CFB-H52H3) is expressed from human 293 cells (HEK293). It contains AA Thr 26 - Leu 764 (Accession # P00751-1).

    Predicted N-terminus: Thr 26

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    Complement Factor B Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 84.9 kDa. The protein migrates as 90-120 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    Complement Factor B SDS-PAGE

    Human Complement Factor B Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Complement Factor B SEC-MALS

    The purity of Human Complement Factor B Protein, His Tag (Cat. No. CFB-H52H3) is more than 90% and the molecular weight of this protein is around 80-115 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-SPR

     Complement Factor B SPR

    Human Complement Factor B Protein, His Tag (Cat. No. CFB-H52H3) immobilized on CM5 Chip can bind Iptacopan (LNP023) with an affinity constant of 20.7 nM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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背景介绍

Complement factor B, a component of the alternative pathway (AP) of complement activation. Factor B is one of the upstream, proximal components unique to the AP and is essential for the activation of the pathway. It is an integral component that drives the amplification loop. It is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.

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