产品详情
分子别名(Synonym)
Complement C5, C5, CPAMD4
表达区间及表达系统(Source)
Biotinylated Cynomolgus Complement C5 Protein, His,Avitag (CO5-C82E3) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Arg 677 & Met 678 - Cys 1676 (Accession # XP_005580972.1).
Predicted N-terminus: Gln 19 (β chain) & Met 678 (α chain)
Request for sequence蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.9 kDa (β chain) and 116.2 kDa (α chain). The protein migrates as 70 kDa and 120-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
标记(Labeling)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度(Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
纯度(Purity)
>95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
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数据展示
电泳(SDS-PAGE)

Biotinylated Cynomolgus Complement C5 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Biotinylated Cynomolgus Complement C5 Protein, His,Avitag (Cat. No. CO5-C82E3) is more than 85% and the molecular weight of this protein is around 170-210 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA

Immobilized Monoclonal Anti-Human C5a Human Antibody, Human IgG1 (Cat. No. CO5-M50) at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus Complement C5 Protein, His,Avitag (Cat. No. CO5-C82E3) with a linear range of 1-63 ng/mL (QC tested).
Protocol
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