Biotinylated Human CLEC12A / MICL / CLL-1 Protein, His,Avitag™ (C-terminus) (MALS verified)

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Qty.
CLA-H82E9-25ug
¥3500.00
CLA-H82E9-200ug
¥13800.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    CLEC12A,MICL,CLL-1,CLL1,DCAL2,DCAL-2,CD371

  • 表达区间及表达系统(Source)

    Biotinylated Human CLEC12A Protein, His,Avitag (CLA-H82E9) is expressed from human 293 cells (HEK293). It contains AA His 65 - Ala 265 (Accession # Q5QGZ9-2).

    Predicted N-terminus: His 65

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    CLEC12A Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 27.3 kDa. The protein migrates as 35-53 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 标记(Labeling)

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • 蛋白标记度(Protein Ratio)

    Passed as determined by the HABA assay / binding ELISA.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    CLEC12A SDS-PAGE

    Biotinylated Human CLEC12A Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    CLEC12A SEC-MALS

    The purity of Biotinylated Human CLEC12A Protein, His,Avitag (Cat. No. CLA-H82E9) is more than 90% and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     CLEC12A ELISA

    Immobilized Biotinylated Human CLEC12A Protein, His,Avitag (Cat. No. CLA-H82E9) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human CLEC12A Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).

    Protocol

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背景介绍

CLEC12A (C-type lectin domain family 12 member A) is also known as CLL1, DCAL2, MICL. Clec12a is an inhibitory receptor for uric acid crystals that regulates inflammation in response to cell death. Cell surface receptor that modulates signaling cascades and mediates tyrosine phosphorylation of target MAP kinases. Evidence of distinct disease propagating stem cells in myelodysplastic syndrome (MDS) has emerged in recent years. The role of CLEC12A in MDS, however, remains to be elucidated. Furthermore, CLEC12A has been proposed as a promising marker of leukaemic stem cells in AML.

前沿进展

 
药物研发进展
  • 英文全称:

    C-Type lectin domain family 12 member A

  • 中文全称:

    C型凝集素结构域家族12成员A

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    19 详情

  • 最高研发阶段:

    临床二期

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