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应用说明（Application）
- Immunization
- FACS
- Elisa
分子别名（Synonym）
Claudin-6, CLDN6
表达区间及表达系统（Source）
Biotinylated Human Claudin-6 Full Length Protein (VLP) (CL6-H82P3) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Val 220 (Accession # P56747).
Predicted N-terminus: Met 1
蛋白结构（Molecular Characterization）
Structure
Virus-like particles (VLPs) are protein particles that mimic the shape of viruses but lack a viral genome, and incorporating transmembrane proteins on the external surface.
Synthesis Process
Virus-like particles (VLPs) are non-infectious structures that mimic the shape of viruses but lack a viral genome. During VLP production in cells systems, VLPs self-assemble into 100-200 nm protein particles, and incorporate transmembrane proteins into a VLP-MP complex, resulting in presentation of the protein on the external surface of the VLP. Their repetitive, multivalent surface display and strong immunogenicity, making them an excellent candidate for immunization.
标记（Labeling）
The VLP is biotinylated with propriety technology.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
制剂（Formulation）
The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Supplied as 0.2 μm filtered solution in PBS, Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 12 months under sterile conditions.
质量管理控制体系（QMS）
- *The isotype control of empty/mock VLP (Cat. No. VLP-N82P3) is sold separately and not included in protein, you can follow the link for product information.
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数据展示
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human Claudin-6 Full Length Protein (VLP) (Cat. No. CL6-H82P3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-Human Claudin-6 antibody, Human IgG1 with a linear range of 0.05-2 ng/mL (QC tested).
Protocol
ELISA comparison of biotinylated full-length human Claudin-6 (virus-like particles) using anti-Claudin-6 antibody shows ACRO (Cat. No. CL6-H82P3) has superior bioactivity to Company K (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Biotinylated Human Claudin-6 Full Length Protein (VLP) (Cat. No. CL6-H82P3) immobilized on SA Chip can bind Anti-Claudin-6 Antibody, Human IgG1 with an affinity constant of 0.283 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
均一性（Identity）-DLS
The mean peak Radius of VLP is 70-90 nm with more than 95% intensity as determined by dynamic light scattering (DLS).
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