产品详情
分子别名(Synonym)
C7orf1, CPE-R2, CPETR2, HRVP1, RVP1
表达区间及表达系统(Source)
Biotinylated Human Claudin-3 Full Length Protein (VLP) (CL3-H82P3) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Val 220 (Accession # O15551).
Predicted N-terminus: Met 1
蛋白结构(Molecular Characterization)
Virus-like particles(VLPs) are formed by self-assembly of capsid proteins from viruses. Membrane Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening.
The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.
制剂(Formulation)
The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Supplied as 0.2 μm filtered solution in PBS, Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
运输(Shipping)
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储(Storage)
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 12 months under sterile conditions.
质量管理控制体系(QMS)
- *The isotype control of empty/mock VLP (Cat. No. VLP-N82P3) is sold separately and not included in protein, you can follow the link for product information.
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数据展示
活性(Bioactivity)-ELISA

Immobilized Biotinylated Human Claudin-3 Full Length Protein (VLP) (Cat. No. CL3-H82P3) at 5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-Claudin 3 Antibody, Human IgG1 with a linear range of 0.5-63 ng/mL (QC tested).
Protocol
均一性(Identity)-DLS

The mean peak Radius of VLP is 60-80 nm with more than 95% intensity as determined by dynamic light scattering (DLS).
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