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应用说明（Application）
- Immunization
- Cell based assay (FACS or functional assay)
- Screening (ELISA)
分子别名（Synonyms）
Claudin-18.2, CLDN18, Claudin-18
表达区间及表达系统（Source）
Biotinylated Human Claudin-18.2 Full Length Protein (VLP) (CL2-H82P3) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Val 261 (Accession # P56856-2 ).
Predicted N-terminus: Met 1
蛋白结构（Molecular Characterization）
*The isotype control of empty/mock VLP (Cat. No. VLP-N82P3) is sold separately and not included in protein, you can follow the link for product information.
Structure
Virus-like particles (VLPs) are protein particles that mimic the shape of viruses but lack a viral genome, and incorporating transmembrane proteins on the external surface.
Synthesis Process
Envelope virus-like particles (eVLPs) are produced by co-expressing target membrane proteins with viral structural proteins in mammalian cells, enabling native embedding of membrane proteins into the lipid bilayer during VLP budding. This approach preserves authentic conformation, post-translational modifications, and orientation, while creating a completely detergent-free environment with repetitive multivalent display and strong immunogenicity making them ideal for immunization, binding assays in antibody discovery and screening (ELISA), and cell-based assays such as FACS, etc.
标记（Labeling）
The VLP is biotinylated with propriety technology.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
纯度（Purity）
>95% as determined by SEC-HPLC.
制剂（Formulation）
The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Supplied as 0.2 μm filtered solution in PBS, Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 12 months under sterile conditions.
质量管理控制体系（QMS）
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数据展示
SEC-HPLC
The purity of Biotinylated Human Claudin-18.2 Full Length Protein (VLP) (Cat. No. CL2-H82P3) was greater than 95% as determined by SEC-HPLC.
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human Claudin-18.2 Full Length Protein (VLP) (Cat. No. CL2-H82P3) at 5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-Claudin-18.2 antibody with a linear range of 0.05-2 ng/mL (QC tested).
Protocol
ELISA comparison of biotinylated human Claudin-18.2 (virus-like particles) using anti-Claudin-18.2 antibody shows ACRO (Cat. No. CL2-H82P3) has superior bioactivity to Company K (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Biotinylated Human Claudin-18.2 Full Length Protein (VLP) (Cat. No. CL2-H82P3) immobilized on SA Chip can bind Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 with an affinity constant of 0.601 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
均一性（Identity）-DLS
The mean peak Radius of VLP is 60-85 nm with more than 95% intensity as determined by dynamic light scattering (DLS).
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