Human CGRPR&RAMP1 Full Length Protein, His,Flag Tag&Twin-Strep Tag (Detergent)

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CG1-H52D3-20ug
¥7350.00
CG1-H52D3-100ug
¥27300.00
CG1-H52D3-500ug
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产品详情

  • 分子别名(Synonym)

    CGRPR & RAMP1

  • 表达区间及表达系统(Source)

    Human CGRPR&RAMP1 Full Length Protein, His,Flag Tag&Twin-Strep Tag (CG1-H52D3) is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Asn 461 & Cys 27 - Val 148 (Accession # Q16602-1 & O60894-1).

    Predicted N-terminus: Glu 23 & Trp

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    CGRPR & RAMP1 Structure

    The CGRPR protein carries a polyhistidine tag and a Flag tag at the C-terminus. The RAMP1 protein carries a Twin Strep tag at the N-terminus. The protein has a calculated MW of 69 kDa.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    This product is not suitable for cell based experiments due to cytotoxicity of DDM.
    DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumstances should you remove DDM and CHS.
    DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
    If glycerol is not compatible with your application, remove glycerol just before the immediate experiment, and NEVER store glycerol-free protein solution.

    Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, pH7.5, CHS with glycerol as protectant.

    Contact us for customized product form or formulation.

  • 运输(Shipping)

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储(Storage)

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程
  • *The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow this link for product information.

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数据展示

  • 活性(Bioactivity)-ELISA

     CGRPR & RAMP1 ELISA

    Immobilized Human CGRPR&RAMP1 Full Length Protein, His,Flag Tag&Twin-Strep Tag (Cat. No. CG1-H52D3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CGRPR antibody, Human IgG1 with a linear range of 0.039-1.25 ng/mL (QC tested).

    Protocol
  • 活性(Bioactivity)-SPR

     CGRPR & RAMP1 SPR

    Anti-CGRPR (Erenumab) captured on Protein A Chip can bind Human CGRPR&RAMP1 Full Length Protein, His,Flag Tag&Twin-Strep Tag (Cat. No. CG1-H52D3) with an affinity constant of 6.92 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).

    Protocol

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背景介绍

This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.

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