产品详情
分子别名(Synonym)
CEACAM8,CD66b,CD67,CGM6,NCA-95
表达区间及表达系统(Source)
Biotinylated Human CEACAM-8, His,Avitag (CE8-H82E9) is expressed from human 293 cells (HEK293). It contains AA Gln 35 - Ser 319 (Accession # P31997-1).
Predicted N-terminus: Gln 35
Request for sequence蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 35.1 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记(Labeling)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度(Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
纯度(Purity)
>95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
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数据展示
电泳(SDS-PAGE)

Biotinylated Human CEACAM-8, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性(Bioactivity)-ELISA

Immobilized Human CEACAM-6, His Tag (Cat. No. CE6-H5223) at 10 μg/mL (100 μL/well) can bind Biotinylated Human CEACAM-8, His,Avitag (Cat. No. CE8-H82E9) with a linear range of 0.02-0.313 μg/mL (Routinely tested).
Protocol
活性(Bioactivity)-SPR

Biotinylated Human CEACAM-8, His,Avitag (Cat. No. CE8-H82E9) immobilized on SA Chip can bind Human CEACAM-6, His Tag (Cat. No. CE6-H5223) with an affinity constant of 4.57 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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