Biotinylated Human CEACAM-8 / CD66b Protein, His,Avitag™ (SPR verified)

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货号-规格
价格
Qty.
CE8-H82E9-25ug
¥3675.00
CE8-H82E9-200ug
¥14490.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    CEACAM8,CD66b,CD67,CGM6,NCA-95

  • 表达区间及表达系统(Source)

    Biotinylated Human CEACAM-8, His,Avitag (CE8-H82E9) is expressed from human 293 cells (HEK293). It contains AA Gln 35 - Ser 319 (Accession # P31997-1).

    Predicted N-terminus: Gln 35

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    CEACAM-8 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 35.1 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 标记(Labeling)

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • 蛋白标记度(Protein Ratio)

    Passed as determined by the HABA assay / binding ELISA.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    CEACAM-8 SDS-PAGE

    Biotinylated Human CEACAM-8, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • 活性(Bioactivity)-ELISA

     CEACAM-8 ELISA

    Immobilized Human CEACAM-6, His Tag (Cat. No. CE6-H5223) at 10 μg/mL (100 μL/well) can bind Biotinylated Human CEACAM-8, His,Avitag (Cat. No. CE8-H82E9) with a linear range of 0.02-0.313 μg/mL (Routinely tested).

    Protocol
  • 活性(Bioactivity)-SPR

     CEACAM-8 SPR

    Biotinylated Human CEACAM-8, His,Avitag (Cat. No. CE8-H82E9) immobilized on SA Chip can bind Human CEACAM-6, His Tag (Cat. No. CE6-H5223) with an affinity constant of 4.57 μM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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背景介绍

Carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8) is also known as CD66b (Cluster of Differentiation 66b), CD66b, CD67, CGM6, NCA-95, and is one of seven human CEACAM family members within the immunoglobulin superfamily. CEACAM family members are a set of widely expressed proteins involved in several biological functions, including cell adhesion, migration, signal transduction, and the regulation of gene expression. Abnormal overexpression and downregulation of some CEACAMs have been described in tumor cells. In humans, CEACAMs include type I transmembrane proteins (CEACAM1, CEACAM3, and CEACAM4) and GPI-linked molecules (CEACAM5 through CEACAM8). There is no human CEACAM2. CEACAM8 is a single chain, two Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. It is an activation marker for human granulocytes.

前沿进展

 
药物研发进展
  • 英文全称:

    Non specific cross-reacting antigen 95

  • 中文全称:

    非特异性交叉反应抗原95

  • 种类:

  • 上市药物数量:

    1 详情

  • 临床药物数量:

    1 详情

  • 最高研发阶段:

    批准上市

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