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分子别名（Synonym）
CEACAM1,CD66a,BGP,BGP1,BGPI
表达区间及表达系统（Source）
Biotinylated Human CEACAM-1, Avitag,His Tag (CE1-H82E5) is expressed from human 293 cells (HEK293). It contains AA Gln 35 - Gly 428 (Accession # P13688-1).
Predicted N-terminus: Gln 35
Request for sequence蛋白结构（Molecular Characterization）
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 45.9 kDa. The protein migrates as 66-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Biotinylated Human CEACAM-1, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized Human CD209, His Tag (Cat. No. CD9-H5246) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CEACAM-1, Avitag,His Tag (Cat. No. CE1-H82E5) with a linear range of 10-78 ng/mL (QC tested).
Protocol
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