Biotinylated Human CD98 Protein, Avitag™,His Tag (MALS verified)

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货号-规格
价格
Qty.
CD8-H82Q3-25ug
¥3200.00
CD8-H82Q3-200ug
¥13785.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    SLC3A2, CD98, 4F2, 4F2HC, 4T2HC, CD98HC, MDU1, NACAE

  • 表达区间及表达系统(Source)

    Biotinylated Human CD98 Protein, Avitag,His Tag (CD8-H82Q3) is expressed from human 293 cells (HEK293). It contains AA Arg 206 - Ala 630 (Accession # P08195-1).

    Predicted N-terminus: Arg 206

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    CD98 Structure

    This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 50.4 kDa. The protein migrates as 60-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 标记(Labeling)

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • 蛋白标记度(Protein Ratio)

    Passed as determined by the HABA assay / binding ELISA.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    CD98 SDS-PAGE

    Biotinylated Human CD98 Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    CD98 SEC-MALS

    The purity of Biotinylated Human CD98 Protein, Avitag,His Tag (Cat. No. CD8-H82Q3) is more than 90% and the molecular weight of this protein is around 50-75 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     CD98 ELISA

    Immobilized Biotinylated Human CD98 Protein, Avitag,His Tag (Cat. No. CD8-H82Q3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind CD98 Recombinant Rabbit Monoclonal Antibody (065) with a linear range of 0.08-1 ng/mL (QC tested).

    Protocol

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背景介绍

CD antigen CD98 is also known as 4F2 cell-surface antigen heavy chain (4F2hc), 4F2 heavy chain antigen, Solute carrier family 3 member 2 (SLC3A2), Lymphocyte activation antigen 4F2 large subunit, is a single-pass type I I membrane protein which belongs to the SLC3A transporter family.. SLC3A2 / CD98 is expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. SLC3A2 / CD98 is required for the function of light chain amino-acid transporters and also involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. CD98 involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A5 or SLC7A8, CD98 involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals.

前沿进展

 
药物研发进展
  • 英文全称:

    L-type amino acid transporter 1

  • 中文全称:

    L-型氨基酸转运蛋白-1

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    7 详情

  • 最高研发阶段:

    申请上市

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