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分子别名（Synonym）
CD8A, CD8, Leu2, MAL, p32
表达区间及表达系统（Source）
Human CD8 alpha Protein, Fc Tag (CDA-H5258) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Asp 182 (Accession # P01732-1).
Predicted N-terminus: Ser 22
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 44.1 kDa. The protein migrates as 53-58 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Human CD8 alpha Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human CD8 alpha Protein, Fc Tag (Cat. No. CDA-H5258) is more than 95% and the molecular weight of this protein is around 90-120 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human CD8 alpha Protein, Fc Tag (Cat. No. CDA-H5258) at 1 μg/mL (100 μL/well) can bind Mouse Anti-CD8 alpha Antibody, Mouse IgG2a with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
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