关注公众号
收藏产品 产品详情
分子别名（Synonym）
CD7, GP40, TP41, LEU-9, Tp40
表达区间及表达系统（Source）
Biotinylated Human CD7 Protein, His,Avitag, premium grade (CD7-H82E4) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Pro 180 (Accession # P09564-1).
Predicted N-terminus: Ala 26
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage.
Request for sequence蛋白结构（Molecular Characterization）
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 20.0 kDa. The protein migrates as 28-38 kDa under reducing (R) condition, and 26-36 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method / rFC method.
宿主蛋白残留（Host Cell Protein）
<0.5 ng/µg of protein tested by ELISA.
宿主核酸残留（Host Cell DNA）
<0.02 ng/μg of protein tested by qPCR.
无菌（Sterility）
Negative
支原体（Mycoplasma）
Negative
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
推荐产品
数据展示
电泳（SDS-PAGE）
Biotinylated Human CD7 Protein, His,Avitag, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human CD7 Protein, His,Avitag, premium grade (Cat. No. CD7-H82E4) is more than 90% and the molecular weight of this protein is around 52-62 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-FACS
2e5 of Anti-CD7 CAR-293 cells were stained with 100 μL of 3 μg/mL of Biotinylated Human CD7 Protein, His,Avitag, premium grade (Cat. No. CD7-H82E4) and negative control protein respectively, washed and then followed by PE-Streptavidin and analyzed with FACS (QC tested).
Protocol
活性（Bioactivity）-ELISA
Immobilized Anti-CD7 antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human CD7 Protein, His,Avitag, premium grade (Cat. No. CD7-H82E4) with a linear range of 0.5-8 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human CD7 Protein, His,Avitag, premium grade (Cat. No. CD7-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-CD7 antibody, Mouse IgG1 with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
用户评价 发表评论
药物研发进展 
联系我们 
项目合作 