Biotinylated Mouse CD47 Protein, His,Avitag™ (MALS verified)

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CD7-M82E4-25ug
¥3675.00
CD7-M82E4-200ug
¥14490.00
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产品详情

  • 分子别名(Synonym)

    CD47, MER6, IAP, OA3

  • 表达区间及表达系统(Source)

    Biotinylated Mouse CD47, His,Avitag (CD7-M82E4) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Lys 140 (Accession # Q61735-1).

    Predicted N-terminus: Gln 19

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    CD47 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 17.4 kDa. The protein migrates as 30-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 标记(Labeling)

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • 蛋白标记度(Protein Ratio)

    Passed as determined by the HABA assay / binding ELISA.

  • 内毒素(Endotoxin)

    Less than 0.1 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    CD47 SDS-PAGE

    Biotinylated Mouse CD47, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    CD47 SEC-MALS

    The purity of Biotinylated Mouse CD47, His,Avitag (Cat. No. CD7-M82E4) is more than 95% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     CD47 ELISA

    Immobilized Biotinylated Mouse CD47, His,Avitag (Cat. No. CD7-M82E4) at 2 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Mouse SIRP alpha, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. SIA-M5252) with a linear range of 0.019-0.625 μg/mL (QC tested).

    Protocol

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背景介绍

Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.

前沿进展

 
药物研发进展
  • 英文全称:

    Cluster of differentiation 47

  • 中文全称:

    分化群47

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    57 详情

  • 最高研发阶段:

    临床二期

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