产品详情
分子别名(Synonym)
CD47,MER6,IAP,OA3
表达区间及表达系统(Source)
Canine CD47, His Tag (CD7-C52H5) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Asn 140 (Accession # F1P6D7-1).
Predicted N-terminus: Gln 19
Request for sequence蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 15.8 kDa. The protein migrates as 27-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度(Purity)
>90% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
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数据展示
电泳(SDS-PAGE)

Canine CD47, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性(Bioactivity)-ELISA

Immobilized Human SIRP alpha, Fc Tag (HPLC-verified) (Cat. No. SIA-H5251) at 5 μg/mL (100 μL/well) can bind Canine CD47, His Tag (Cat. No. CD7-C52H5) with a linear range of 5-78 ng/mL (QC tested).
Protocol
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