Mouse CD45 / PTPRC protein, His Tag (MALS & SPR verified)

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CD5-M52H3-100ug
¥3850.00
CD5-M52H3-1mg
¥23100.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    CD45,PTPRC,L-CA,T200

  • 表达区间及表达系统(Source)

    Mouse CD45 protein, His Tag (CD5-M52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Lys 566 (Accession # P06800-4 ).

    Predicted N-terminus: Gln 26

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    CD45 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 60.6 kDa. The protein migrates as 100-135 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    CD45 SDS-PAGE

    Mouse CD45 protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    CD45 SEC-MALS

    The purity of Mouse CD45 protein, His Tag (Cat. No. CD5-M52H3) is more than 90% and the molecular weight of this protein is around 90-120 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-SPR

     CD45 SPR

    Mouse CD45 protein, His Tag (Cat. No. CD5-M52H3) captured on CM5 chip via anti-His antibody can bind Human Galectin-1, Tag Free (Cat. No. GA1-H4113) with an affinity constant of 7.19 µM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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背景介绍

CD45 is a receptor protein tyrosine phosphatase, also known as Ly-5 or leukocyte common antigen. CD45 mainly involves in the initiation of T cell receptor signaling by controlling the activation of the Src family protein-tyrosine kinases Lck and Fyn. CD45 deficiency causes in T- and B-lymphocyte dysfunction in the form of severe combined immune deficiency. It also takes a significant role in autoimmune diseases and cancer as well as in infectious diseases including fungal infections.

前沿进展

 
药物研发进展
  • 英文全称:

    Leukocyte common antigen

  • 中文全称:

    白细胞共同抗原

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    3 详情

  • 最高研发阶段:

    临床三期

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