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分子别名（Synonym）
CD4, CD4mut, LEU3
表达区间及表达系统（Source）
Alexa Fluor 488-Labeled Human CD4 Protein, His Tag (CD4-HA225) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Trp 390 (Accession # AAH25782). It is the Alexa Fluor™ 488 labeled form of Human CD4, His Tag (Cat. No. LE3-H5228).
Predicted N-terminus: Lys 26
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 41.5 kDa.
偶联（Conjugate）
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
标记（Labeling）
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with AF488 using standard chemical labeling method. The residual AF488 is removed by molecular sieve treatment during purification process.
蛋白标记度（Protein Ratio）
The AF488 to protein molar ratio is 2-4.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
活性（Bioactivity）-ELISA
Immobilized Alexa Fluor 488-Labeled Human CD4 Protein, His Tag (Cat. No. CD4-HA225) at 1 μg/mL (100 μL/well) can bind Anti-CD4 Antibody (Ibalizumab-IgG4) with a linear range of 0.1-8 ng/mL (Routinely tested). Labeling with fluorescent dyes did not affect their activity.
Protocol
活性（Bioactivity）-FACS
1e5 of Mouse Anti-CD4 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD4 Protein, His Tag (Cat. No. CD4-HA225) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
Protocol
1e5 of Mouse Anti-CD4 antibody coupled beads (5.5 μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD4 Protein, His Tag (Cat. No. CD4-HA225) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
Protocol
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