Mouse CD3 epsilon&CD3 delta Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified)

分子别名（Synonym）

CD3E & CD3D, CD3 delta & CD3 epsilon

表达区间及表达系统（Source）

Mouse CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (CDD-M52W2) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 108 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P22646-1 (CD3E) & P04235-1 (CD3D)).

Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)

蛋白结构（Molecular Characterization）

Mouse CD3E&CD3D Heterodimer Protein, Fc, His Tag&Fc, Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 41.5 kDa (CD3E) and 40.7 kDa (CD3D). Subunit CD3E is fused with a human IgG1 Fc tag and a polyhistidine tag at the C-terminus and subunit CD3D is fused with a human IgG1 Fc tag and a flag tag at the C-terminus. As a result of glycosylation, the heterodimer protein migrates as 55-65 kDa under reducing (R) condition, and 90-116 kDa under non-reducing (NR) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程