Alexa Fluor™ 488-Labeled Human CD3 epsilon & CD3 delta Heterodimer Protein, His Tag&Tag FreeStar Staining

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CDD-HA2W5-25ug
¥4500.00
CDD-HA2W5-200ug
¥28030.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    CD3E & CD3D, CD3 delta & CD3 epsilon

  • 表达区间及表达系统(Source)

    Alexa Fluor 488-Labeled Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (CDD-HA2W5) is produced via conjugation of AF488 to Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free with a new generation site-specific technology under Star Staining labeling platform. Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1 (CD3E) & P04234-1 (CD3D)).

    Predicted N-terminus: Asp 23 & Phe 22

  • 蛋白结构(Molecular Characterization)

    Alexa Fluor 488-Labeled Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free, produced by co-expression of CD3E and CD3D. Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D contains no tag.

  • 偶联(Conjugate)

    AF488

    Excitation Wavelength: 488 nm

    Emission Wavelength: 517 nm

  • 蛋白标记度(Protein Ratio)

    The AF488 to protein molar ratio is 0.9-1.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 活性(Bioactivity)-ELISA

     CD3E & CD3D ELISA

    Immobilized Alexa Fluor 488-Labeled Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-HA2W5) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) (Cat. No. CDE-M531) with a linear range of 0.1-8 ng/mL (Routinely tested). Labeling with fluorescent dyes did not affect their activity.

    Protocol
  • 活性(Bioactivity)-FACS

     CD3E & CD3D FACS

    1e5 of ActiveMax® Human T cell Activation/Expansion CD3/CD28 Beads, premium grade were stained with different concentration of Alexa Fluor 488-Labeled Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-HA2W5) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).

    Protocol
  • 稳定性(Stability)

     CD3E & CD3D STABILITY

    Alexa Fluor 488-Labeled Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-HA2W5) is stable at 25℃ for 50 hours, equivalent to store at -70 ℃ for 2 years and freezing and thawing 3 times without performance reduction.

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背景介绍

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

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