产品详情
分子别名(Synonym)
CD3E & CD3D, CD3 delta & CD3 epsilon
表达区间及表达系统(Source)
Alexa Fluor 488-Labeled Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (CDD-HA2W5) is produced via conjugation of AF488 to Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free with a new generation site-specific technology under Star Staining labeling platform. Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1 (CD3E) & P04234-1 (CD3D)).
Predicted N-terminus: Asp 23 & Phe 22
蛋白结构(Molecular Characterization)
Alexa Fluor 488-Labeled Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free, produced by co-expression of CD3E and CD3D. Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D contains no tag.
偶联(Conjugate)
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
蛋白标记度(Protein Ratio)
The AF488 to protein molar ratio is 0.9-1.
纯度(Purity)
>90% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
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数据展示
活性(Bioactivity)-ELISA

Immobilized Alexa Fluor 488-Labeled Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-HA2W5) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) (Cat. No. CDE-M531) with a linear range of 0.1-8 ng/mL (Routinely tested). Labeling with fluorescent dyes did not affect their activity.
Protocol
活性(Bioactivity)-FACS

1e5 of ActiveMax® Human T cell Activation/Expansion CD3/CD28 Beads, premium grade were stained with different concentration of Alexa Fluor 488-Labeled Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-HA2W5) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
Protocol
稳定性(Stability)

Alexa Fluor 488-Labeled Human CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-HA2W5) is stable at 25℃ for 50 hours, equivalent to store at -70 ℃ for 2 years and freezing and thawing 3 times without performance reduction.
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