Canine CD3 epsilon & CD3 delta Heterodimer Protein, His Tag&Tag Free (MALS verified)

分子别名（Synonym）

CD3E & CD3D,CD3 delta & CD3 epsilon

表达区间及表达系统（Source）

Canine CD3E & CD3D Heterodimer Protein, His Tag&Tag Free (CDD-C52W3) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Leu 122 (CD3E) & Phe 22 - Thr 103 (CD3D) (Accession # P27597-1 (CD3E) & XP_536556.1 (CD3D)).

Predicted N-terminus: Gln 22 (CD3E) & Phe 22 (CD3D)

蛋白结构（Molecular Characterization）

Canine CD3E & CD3D Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3D, has a calculated MW of 16.6 kDa (CD3E) and 13.3 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D contains no tag. As a result of glycosylation, the heterodimer protein migrates as 19-20 kDa (CD3E) under reducing (R) condition, and 36-45 kDa (CD3D) under non-reducing (NR) condition (SDS-PAGE).

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程