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分子别名（Synonym）
CD38, T10, cADPr hydrolase 1
表达区间及表达系统（Source）
FITC-Labeled Human CD38, His Tag (CD8-HF2H5) is expressed from human 293 cells (HEK293). It contains AA Val 43 - Ile 300 (Accession # P28907-1). It is the FITC labeled form of Human CD38, His Tag (Cat. No. CD8-H5224).
Predicted N-terminus: Val 43
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 30.69 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
标记（Labeling）
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
蛋白标记度（Protein Ratio）
The FITC to protein molar ratio is 2-4.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
FITC-Labeled Human CD38, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of FITC-Labeled Human CD38, His Tag (Cat. No. CD8-HF2H5) is more than 90% and the molecular weight of this protein is around 35-50 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Anti-CD38/Daratumumab, Human IgG1 (CH1/CH3 mutation) at 2 μg/mL (100 μL/well) can bind FITC-Labeled Human CD38, His Tag (Cat. No. CD8-HF2H5) with a linear range of 0.078-5 μg/mL (QC tested).
Protocol
活性（Bioactivity）-FACS
2e5 of Anti-CD38 CAR-293 cells were stained with 100 μL of 10 μg/mL of FITC-Labeled Human CD38 Protein, His Tag (MALS verified) (Cat. No. CD8-HF2H5) and negative control protein respectively. FITC signal was used to evaluate the binding activity (Routinely tested).
Protocol
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