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分子别名（Synonym）
CD38, T10, cADPr hydrolase 1
表达区间及表达系统（Source）
Human CD38, Fc Tag (CD8-H5255) is expressed from human 293 cells (HEK293). It contains AA Val 43 - Ile 300 (Accession # P28907-1).
Predicted N-terminus: Val 43
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 56.5 kDa. The protein migrates as 66-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Human CD38, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human CD38, Fc Tag (Cat. No. CD8-H5255) is more than 95% and the molecular weight of this protein is around 115-145 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human CD38, Fc Tag (Cat. No. CD8-H5255) at 2 μg/mL (100 μL/well) can bind Anti-CD38, Human IgG1 with a linear range of 0.3-10 ng/mL (QC tested).
Protocol
活性（Bioactivity）-FACS
2e5 of Anti-CD38 CAR-293 cells were stained with 100 μL of 3 μg/mL of Human CD38 Protein, Fc Tag (Cat. No. CD8-H5255) and negative control protein respectively, washed and then followed by PE anti-human IgG Fc antibody and analyzed with FACS (Routinely tested).
Protocol
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