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分子别名（Synonym）
FLJ18683, T3E, TCRE, CD3E, CD3-epsilon
表达区间及表达系统（Source）
Human CD3 epsilon (23-48) Protein, Fc Tag (CDE-H5253) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Thr 48 (Accession # P07766).
Predicted N-terminus: Asp 23
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 29.2 kDa. The protein migrates as 40-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Human CD3 epsilon (23-48) Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human CD3 epsilon (23-48) Protein, Fc Tag (Cat. No. CDE-H5253) is more than 90% and the molecular weight of this protein is around 65-80 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human CD3 epsilon (23-48) Protein, Fc Tag (Cat. No. CDE-H5253) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) (Cat. No. CDE-M531) with a linear range of 0.06-4 ng/mL (QC tested).
Protocol
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背景介绍
Its primary function is to act as a crucial structural scaffold, mediating proper interactions with other CD3 subunits (specifically CD3G and CD3D).
It is considered as SP34 binding site.
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