Alexa Fluor™ 488-Labeled Human CD27 Ligand / CD70 Protein, His TagStar Staining

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CDL-HA247-25ug
¥4410.00
CDL-HA247-200ug
¥27470.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    CD70, CD27LG, TNFSF7, TNFSF7G, CD27L

  • 表达区间及表达系统(Source)

    Alexa Fluor 488-Labeled Human CD27 Ligand Protein, His Tag (CDL-HA247) is produced via conjugation of AF488 to Human CD27 Ligand Protein, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human CD27 Ligand Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 52 - Pro 193 (Accession # P32970-1).

    Predicted N-terminus: His

  • 蛋白结构(Molecular Characterization)

    This protein carries a polyhistidine tag at the N-terminus.

  • 偶联(Conjugate)

    AF488

    Excitation Wavelength: 488 nm

    Emission Wavelength: 517 nm

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • CAR阳性表达率检测(Evaluation of CAR expression)

    FACS Analysis of Anti-CD27 Ligand CAR Expression

     CD27 Ligand CAR_T

    5e5 of anti-CD27 Ligand CAR-293 cells were stained with 100 μL of 3 μg/mL of Alexa Fluor 488-Labeled Human CD27 Ligand Protein, His Tag (Cat. No. CDL-HA247) and negative control protein respectively (Fig. C and B), and non- transfected 293 cells were used as a control (Fig. A), Alexa Fluor 488 signal was used to evaluate the binding activity (QC tested).

    Protocol

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背景介绍

Cluster of Differentiation 70 (CD70) is also known as CD27 ligand (CD27L / CD27LG), TNFSF7, TNFSF7G, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and is a surface antigen found on activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T-cells, and thus is invloved in the biological processes including T-cell activation, the proliferation of costimulated T-cells, as well as the generation of cytolytic T-cells. CD70 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. CD70 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses. CD70 induces proliferation and IFNγ production, on NK cells.

前沿进展

 
药物研发进展
  • 英文全称:

    CD70 antigen

  • 中文全称:

    CD70抗原

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    30 详情

  • 最高研发阶段:

    临床二期

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