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分子别名（Synonym）
MMR, CD206, hMR, MRC1, CLEC13D, CLEC13DL, MRC1L1
表达区间及表达系统（Source）
Biotinylated Human CD206 Protein, His Tag, primary amine labeling (CD6-H82H6) is expressed from human 293 cells (HEK293). It contains AA Leu 19 - Ala 1389 (Accession # P22897-1). It is the biotinylated form of Human CD206, His Tag (Cat. No. CD6-H52H9).
Predicted N-terminus: Leu 19
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 158.6 kDa. The protein migrates as 170-210 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Biotinylated Human CD206 Protein, His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Biotinylated Human CD206 Protein, His Tag, primary amine labeling (Cat. No. CD6-H82H6) is more than 90% and the molecular weight of this protein is around 180-210 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Mannan from Saccharomyces cerevisiae at 20 μg/mL (100 μL/well) can bind Biotinylated Human CD206 Protein, His Tag, primary amine labeling (Cat. No. CD6-H82H6) with a linear range of 0.156-2.5 μg/mL (QC tested).
Protocol
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