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分子别名（Synonym）
MS4A1, CD20, MS4A-1
表达区间及表达系统（Source）
Biotinylated Human CD20 Full Length Protein, Flag Tag&His,Avitag (CD0-H82D3) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Pro 297 (Accession # P11836-1).
Predicted N-terminus: Met
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus followed by an Avi tag (Avitag™). The protein has a calculated MW of 39.1 kDa.
Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by an amphipathic scaffold coat that confers enhanced stability and a narrow particle size distribution.
The nanodisc-pro is directly extracted from cell membrane in a water-soluble form using an amphipathic scaffold followed by affinity chromatography, and thus, it conserves a patch of native membrane around them.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
纯度（Purity）
>85% as determined by SDS-PAGE.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
质量管理控制体系（QMS）
- *The isotype control of empty/mock nanodisc (Cat. No. APO-H5143) is sold separately and not included in protein, you can follow this link for product information.
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数据展示
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human CD20 Full Length Protein, Flag Tag&His,Avitag (Cat. No. CD0-H82D3) at 5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Rituximab biosimilar (Cat. No. CD0-M36) with a linear range of 0.6-39 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human CD20 Full Length Protein, Flag Tag&His,Avitag (Cat. No. CD0-H82D3) at 5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Ofatumumab biosimilar with a linear range of 0.001-0.078 μg/mL (QC tested).
Protocol
Immobilized Biotinylated Human CD20 Full Length Protein, Flag Tag&His,Avitag (Cat. No. CD0-H82D3) at 5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Obinutuzumab biosimilar with a linear range of 0.03-1 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Rituximab biosimilar (Cat. No. CD0-M36) captured on Protein A Chip can bind Biotinylated Human CD20 Full Length Protein, Flag Tag&His,Avitag (Cat. No. CD0-H82D3) with an affinity constant of 21.4 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
活性（Bioactivity）-FACS
2e5 of Anti-CD20 CAR-293 cells were stained with 100 μL of 3 μg/mL Biotinylated Human CD20 Full Length Protein, FlagTag&His,Avitag (Cat. No. CD0-H82D3) and negative control protein respectively, washed and then followed by PE-SA and analyzedwith FACS (QC tested).
Protocol
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