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应用说明（Application）
分子别名（Synonym）
MS4A1, CD20, MS4A-1
表达区间及表达系统（Source）
Detergent Human CD20 Full Length Protein, His Tag (CD0-H52H3) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Pro 297 (Accession # P11836-1).
Predicted N-terminus: Met 1
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 35.2 kDa. The protein migrates as 40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
*The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow the link for product information.
Structure
Each full-length transmembrane protein is encapsulated by detergent micelle.
Synthesis Process
Through mild detergent micelle extraction, dedicated solubilization, and stringent affinity purification, we maximally retain the native transmembrane conformation of full-length transmembrane proteins throughout downstream purification. This workflow yields purified, detergent-solubilized proteins free of unrelated membrane impurities and exogenous scaffold proteins, with exceptional purity, accurate quantitation, and well-preserved biological activity—ideal for immunization, binding assays in antibody discovery and screening (ELISA) and affinity testing (SPR and BLI), etc.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>85% as determined by SDS-PAGE.
制剂（Formulation）
This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumstances should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible with your application, remove glycerol just before the immediate experiment, and NEVER store glycerol-free protein solution.Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Human CD20 Full Length Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 85% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human CD20 Full Length Protein, His Tag (Cat. No. CD0-H52H3) at 5 μg/mL (100 μL/well) can bind Rituximab (Cat. No. CD0-M36) with a linear range of 0.4-3 ng/mL (QC tested).
Protocol
Immobilized Human CD20 Full Length Protein, His Tag (Cat. No. CD0-H52H3) at 5 μg/mL (100 μL/well) can bind Ofatumumab with a linear range of 0.4-6 ng/mL (QC tested).
Protocol
Immobilized Human CD20 Full Length Protein, His Tag (Cat. No. CD0-H52H3) at 5 μg/mL (100 μL/well) can bind Biosimilar of Obinutuzumab with a linear range of 1-8 ng/mL (Routinely tested).
Protocol
Immobilized Human CD20 Full Length Protein, His Tag (Cat. No. CD0-H52H3 at 1 μg/mL, add Glofitamab and then add Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDG-H82W3) at 1 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (Routinely tested).
Protocol
活性（Bioactivity）-SPR
MabThera® (Rituximab) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human CD20 Full Length Protein, His Tag (Cat. No. CD0-H52H3) with an affinity constant of 6.21 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (QC tested).
Protocol
Ofatumumab captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human CD20 Full Length Protein, His Tag (Cat. No. CD0-H52H3) with an affinity constant of 9.63 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (QC tested).
Protocol
Biosimilar of Obinutuzumab captured on CM5 chip via anti-human IgG Fc antibody can bind Human CD20 Full Length Protein, His Tag (Cat. No. CD0-H52H3) with an affinity constant of 21.5 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore T200) (Routinely tested).
Protocol
Human CD20 Full Length Protein, His Tag (Cat. No. CD0-H52H3) captured on CM5 chip via Anti-His antibody can bind MabThera® (Rituximab) with an affinity constant of 0.921 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Rituximab on AHC Biosensor, can bind Human CD20 Full Length Protein, His Tag (Cat. No. CD0-H52H3) with an affinity constant of 1.14 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Ofatumumab (Human IgG1) on AHC Biosensor, can bind Human CD20 Full Length Protein, His Tag (Cat. No. CD0-H52H3) with an affinity constant of 0.968 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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