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应用说明（Application）
分子别名（Synonyms）
MS4A1, CD20, MS4A-1
表达区间及表达系统（Source）
Nanodisc Canine CD20 Full Length Protein, His Tag (CD0-C52H9) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Pro 297 (Accession # Q3C2E2-1).
Predicted N-terminus: Met 1
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 35.2 kDa. The protein migrates as 25 kDa and 40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
*The isotype control of empty/mock nanodisc (Cat. No. APO-H51H3) is sold separately and not included in protein, you can follow the link for product information.
Structure
Nanodisc membrane protein is wrapped by a synthetic phospholipid bilayer membrane structure, composed of membrane scaffold proteins (MSPs) and phospholipids.
Synthesis Process
Nanodisc reconstitution is a self-assembly process that starts from purified, detergent-solubilized membrane proteins and reconstitutes them into synthetic phospholipid bilayers stabilized by MSP1D1 membrane scaffold protein. This creates a stable, detergent-free environment that preserves native conformation and biological activity, with excellent homogeneity and full compatibility with cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA),etc.
纯度（Purity）
>80% as determined by SDS-PAGE.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
运输和存储（Shipping and Storage）
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Canine CD20 Full Length Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 80%.
活性（Bioactivity）-ELISA
Immobilized Canine CD20 Full Length, His Tag (Nanodisc) (Cat. No. CD0-C52H9) at 5 μg/mL (100 μL/well) can bind Rabbit Anti-CD20 antibody with a linear range of 5-78 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Rabbit anti-Human CD20 Antibody captured on Protein G Chip can bind Canine CD20 Full Length Protein, His Tag (Cat. No. CD0-C52H9) with an affinity constant of 1.58 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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