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分子别名（Synonym）
MS4A1, CD20, MS4A-1
表达区间及表达系统（Source）
Canine CD20 Full Length Protein, His Tag (CD0-C52H9) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Pro 297 (Accession # Q3C2E2-1).
Predicted N-terminus: Met 1
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 35.2 kDa. The protein migrates as 25 kDa and 40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by an amphipathic scaffold coat that confers enhanced stability and a narrow particle size distribution.
The nanodisc assembles from a mixture of full length membrane protein in detergent, phospholipid micelles and membrane scaffold protein upon removal of the detergent.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>80% as determined by SDS-PAGE.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
质量管理控制体系（QMS）
- *The isotype control of empty/mock nanodisc (Cat. No. APO-H51H3) is sold separately and not included in protein, you can follow this link for product information.
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数据展示
电泳（SDS-PAGE）
Canine CD20 Full Length Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 80%.
活性（Bioactivity）-ELISA
Immobilized Canine CD20 Full Length, His Tag (Nanodisc) (Cat. No. CD0-C52H9) at 5 μg/mL (100 μL/well) can bind Rabbit Anti-CD20 antibody with a linear range of 5-78 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Rabbit anti-Human CD20 Antibody captured on Protein G Chip can bind Canine CD20 Full Length Protein, His Tag (Cat. No. CD0-C52H9) with an affinity constant of 1.58 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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