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分子别名（Synonym）
CD2, SRBC, LFA-2, T11
表达区间及表达系统（Source）
Human CD2, His Tag (CD2-H5226) is expressed from human 293 cells (HEK293). It contains AA Lys 25 - Asp 209 (Accession # AAH33583).
Predicted N-terminus: Lys 25
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 22.3 kDa. The protein migrates as 33-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Human CD2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human CD2, His Tag (Cat. No. CD2-H5226) is more than 95% and the molecular weight of this protein is around 23-35 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human CD2, His Tag (Cat. No. CD2-H5226) at 10 μg/mL (100 μL/well) can bind Human CD58, Fc Tag (Cat. No. LF3-H5256) with a linear range of 0.04-0.31 ug/mL (QC tested).
Protocol
活性（Bioactivity）-BLI
Loaded bind Human CD58, Fc Tag (Cat. No. LF3-H5256) on Protein A Biosensor, can bind Human CD2, His Tag (Cat. No. CD2-H5226) with an affinity constant of 0.49 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded bind Human CD58, Fc Tag (Cat. No. LF3-H5256) on AHC Biosensor, can bind Human CD2, His Tag (Cat. No. CD2-H5226) with an affinity constant of 0.57 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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