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分子别名（Synonym）
CD19, B4, CVID3, MGC12802
表达区间及表达系统（Source）
Mouse CD19 Protein, Fc Tag (CD9-M5257) is expressed from human 293 cells (HEK293). It contains AA Arg 19 - Gly 287 (Accession # P25918).
Predicted N-terminus: Arg 19
Request for sequence蛋白结构（Molecular Characterization）
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 56.0 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Mouse CD19 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mouse CD19 Protein, Fc Tag (Cat. No. CD9-M5257) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Rat/IgG2a,Kappa Anti-CD19 Antibody at 0.5 μg/mL (100 μL/well) can bind Mouse CD19 Protein, Fc Tag (Cat. No. CD9-M5257) with a linear range of 1-39 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Rat/IgG2a,Kappa Anti-CD19 Antibody immobilized on CM5 Chip can bind Mouse CD19 Protein, Fc Tag (Cat. No. CD9-M5257) with an affinity constant of 94.2 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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背景介绍
es, NTPDase-3 plays a role in the regulation of purinergic signaling. The enzyme is expressed at its highest levels in brain, pancreas, spleen and prostate tissues.
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