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分子别名（Synonym）
CD133,PROM1,PROML1,Prominin-1,AC133
表达区间及表达系统（Source）
Biotinylated Human CD133 Full Length Protein, His,Flag Tag&Avitag (CD3-H82D3) is expressed from human 293 cells (HEK293). It contains AA Gly 20 - His 865 (Accession # O43490-1).
Predicted N-terminus: Gly 20
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag, followed by a Flag tag and an Avi tag (Avitag™) at the C-terminus. The protein has a calculated MW of 100.6 kDa. The protein migrates as 95-120 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumstances should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible with your application, remove glycerol just before the immediate experiment, and NEVER store glycerol-free protein solution.Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
质量管理控制体系（QMS）
- *The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow this link for product information.
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数据展示
电泳（SDS-PAGE）
Biotinylated Human CD133 Full Length Protein, His,Flag Tag&Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human CD133 Full Length Protein, His,Flag Tag&Avitag (Cat. No. CD3-H82D3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human CD133 Antibody, Human IgG1 with a linear range of 0.06-2 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Monoclonal Anti-Human CD133 Antibody captured on Protein A Chip can bind Biotinylated Human CD133 Full Length Protein, His,Flag Tag&Avitag (Cat. No. CD3-H82D3) with an affinity constant of 27.1 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).
Protocol
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