HIV (HIV1-HXB2) Capsid protein p24, His Tag (MALS verified)

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CP4-H52H3-100ug
¥3990.00
CP4-H52H3-1mg
¥26670.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    Capsid protein p24/Gag p24 (HIV)

  • 表达区间及表达系统(Source)

    HIV (HIV1-HXB2) Capsid protein p24, His Tag (CP4-H52H3) is expressed from human 293 cells (HEK293). It contains AA Pro 133 - Leu 363 (Accession # P04591).

    Predicted N-terminus: Pro 133

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    Capsid protein p24/Gag p24 (HIV) Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 27.5 kDa. The protein migrates as 26-27 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 电泳(SDS-PAGE)

    Capsid protein p24/Gag p24 (HIV) SDS-PAGE

    HIV (HIV1-HXB2) Capsid protein p24, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Capsid protein p24/Gag p24 (HIV) SEC-MALS

    The purity of HIV (HIV1-HXB2) Capsid protein p24, His Tag (Cat. No. CP4-H52H3) is more than 90% and the molecular weight of this protein is around 24-34 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     Capsid protein p24/Gag p24 (HIV) ELISA

    Immobilized HIV (HIV1-HXB2) Capsid protein p24, His Tag (Cat. No. CP4-H52H3) at 1 μg/mL (100 μL/well) can bind HIV1 p24 Monoclonal Antibody ,Mouse IgG2a with a linear range of 0.1-2 ng/mL (QC tested).

    Protocol

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背景介绍

The Gag protein from retroviruses, also known as p24, forms the inner protein layer of the nucleocapsid. It is composed of two domains, the N-terminal domain (NTD), which contributes to viral core formation, and the C-terminal domain (CTD), which is required for capsid dimerisation, Gag oligomerization and viral formation. This protein performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. ELISA tests for p24 is the most commonly used method to demonstrate virus replication both in vivo and in vitro.

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