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分子别名（Synonym）
Cadherin-17, CDH17, HPT-1, LI-cadherin
表达区间及表达系统（Source）
FITC-Labeled Human Cadherin-17 (23-787) Protein, His Tag (CA7-HF2H3) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Met 787 (Accession # Q12864-1). It is the FITC labeled form of Human Cadherin-17 Protein, His Tag (Active) (Cat. No. CA7-H52H4).
Predicted N-terminus: Gln 23
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 86.9 kDa. The protein migrates as 95-110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
偶联（Conjugate）
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
标记（Labeling）
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
FITC-Labeled Human Cadherin-17 (23-787) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized FITC-Labeled Human Cadherin-17 (23-787) Protein, His Tag (Cat. No. CA7-HF2H3) at 1 μg/mL (100 μL/well) can bind various dilution ratio of Anti-Cadherin 17 Antibody (QC tested).
Protocol
活性（Bioactivity）-SPR
Anti-Cadherin 17 Antibody captured on CM5 chip via anti-mouse antibodies surface can bind FITC-Labeled Human Cadherin-17 (23-787) Protein, His Tag (Cat. No. CA7-HF2H3) with an affinity constant of 3.11 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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