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分子别名（Synonym）
Cadherin-17,CDH17,HPT-1,LI-cadherin
表达区间及表达系统（Source）
Biotinylated Human Cadherin-17 Protein, His,Avitag (CA7-H82E3) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Met 787 (Accession # Q12864-1).
Predicted N-terminus: Gln 23
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 88.6 kDa. The protein migrates as 105-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Biotinylated Human Cadherin-17 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human Cadherin-17 Protein, His,Avitag (Cat. No. CA7-H82E3) is more than 90% and the molecular weight of this protein is around 100-140 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Anti-Cadherin 17/CDH17 Antibody, Mouse IgG2a at 0.1% (100 μL/well) can bind Biotinylated Human Cadherin-17 Protein, His,Avitag (Cat. No. CA7-H82E3) with a linear range of 0.2-6 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human Cadherin-17 Protein, His,Avitag (Cat. No. CA7-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind various dilution ratio of Anti-Cadherin 17/CDH17 Antibody, Mouse IgG2a (Routinely tested).
Protocol
Immobilized Biotinylated Human Cadherin-17 Protein, His,Avitag (Cat. No. CA7-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Cadherin-17 Monoclonal antibody, Mouse IgG1 with a linear range of 0.02-5 ng/mL (Routinely tested).
Protocol
Immobilized Biotinylated Human Cadherin-17 Protein, His,Avitag (Cat. No. CA7-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-CDH17 mAb, Humanized IgG with a linear range of 0.02-1 ng/mL (Routinely tested).
Protocol
Immobilized Biotinylated Human Cadherin-17 Protein, His,Avitag (Cat. No. CA7-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-CDH17 antibody, Rabbit IgG with a linear range of 0.1-6 ng/mL (Routinely tested).
Protocol
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