关注公众号
收藏产品 产品详情
分子别名（Synonym）
TNFRSF17, CD269, BCM, BCMA
表达区间及表达系统（Source）
Biotinylated Mouse BCMA, Fc,Avitag (BCA-M82F0) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Thr 49 (Accession # O88472-1).
Predicted N-terminus: Met 1
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 34.4 kDa. The protein migrates as 38-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to O-linked glycosylation which has been verified by O-Glycosidase and Neuraminidase digestion.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
推荐产品
数据展示
电泳（SDS-PAGE）
Biotinylated Mouse BCMA, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Biotinylated Mouse BCMA, Fc,Avitag (Cat. No. BCA-M82F0) was greater than 85% as determined by SEC-HPLC.
活性（Bioactivity）-ELISA
Immobilized Mouse BAFF, Mouse IgG2a Fc Tag (Cat. No. BAF-M5257) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse BCMA, Fc,Avitag (Cat. No. BCA-M82F0) with a linear range of 2-39 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Mouse BCMA, Fc,Avitag (Cat. No. BCA-M82F0) at 1 μg/mL (100 μL/well) on Recombinant Streptavidin (Cat. No. STN-N5116) precoated (0.5μg/well) plate, can bind Human APRIL, His,Flag Tag, active trimer (Cat. No. APL-H52D1) with a linear range of 10-78 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Biotinylated Mouse BCMA, Fc,Avitag (Cat. No. BCA-M82F0) on SA Biosensor, can bind Mouse BAFF, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. BAF-M5257) with an affinity constant of 3.75 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Mouse BCMA, Fc,Avitag (Cat. No. BCA-M82F0) on SA Biosensor, can bind Human APRIL, His,Flag Tag, active trimer (Cat. No. APL-H52D1) with an affinity constant of 2.19 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
用户评价 发表评论
药物研发进展 
联系我们 
项目合作 