Human BAFF & APRIL Heterotrimer Protein, His Tag (MALS verified)

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BAL-H5243-100ug
¥4040.00
BAL-H5243-1mg
¥27300.00
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产品详情

  • 分子别名(Synonym)

    BAFF & APRIL

  • 表达区间及表达系统(Source)

    Human BAFF & APRIL Heterotrimer Protein, His Tag (BAL-H5243) is expressed from human 293 cells (HEK293). It contains AA Glu 140 - Leu 285 & Gln 111 - Leu 250 (Accession # Q9Y275-1 & O75888-1).

    Predicted N-terminus: His

  • 蛋白结构(Molecular Characterization)

    This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 50 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. The protein is a heterotrimer consisting of one BAFF and two APRIL.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in 25 mM MES, 150 mM NaCl, pH6.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    BAFF & APRIL SDS-PAGE

    Human BAFF & APRIL Heterotrimer Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    BAFF & APRIL SEC-MALS

    The purity of Human BAFF & APRIL Heterotrimer Protein, His Tag (Cat. No. BAL-H5243) is more than 90% and the molecular weight of this protein is around 45-65 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     BAFF & APRIL ELISA

    Immobilized Human BCMA, Fc Tag, premium grade (Cat. No. BC7-H5254) at 5 μg/mL (100 μL/well) can bind Human BAFF & APRIL Heterotrimer Protein, His Tag (Cat. No. BAL-H5243) with a linear range of 0.016-0.125 μg/mL (QC tested).

    Protocol
  •  BAFF & APRIL ELISA

    Immobilized Human BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) at 5 μg/mL (100 μL/well) can bind Human BAFF & APRIL Heterotrimer Protein, His Tag (Cat. No. BAL-H5243) with a linear range of 0.016-0.125 μg/mL (Routinely tested).

    Protocol

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背景介绍

BAFF and APRIL are two members of the TNF superfamily that are secreted by activated myeloid cells and have costimulatory activity on B cells. BAFF/APRIL signaling regulates a diverse range of biological processes, including cell differentiation, proliferation, survival, and effector functions. Dysregulation of the BAFF/APRIL system has been linked to several inflammatory conditions, specifically autoimmune diseases. Elevated levels of BAFF and APRIL have been reported in autoimmune SLE patients. Both BAFF and APRIL have been described as homotrimeric molecules, a feature common to members of the TNF superfamily. But APRIL and BAFF can form active heterotrimer molecules when co-expressed, and circulating heterotrimer exists in serum samples of patients with systemic immune rheumatic diseases. These findings increase the possibility that the active BAFF/APRIL heterotrimer may play a role in rheumatic diseases and other autoimmune diseases.

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