选择语言
免费注册
关注公众号
微信客服
收藏产品

Mouse B7-2 / CD86 Protein, Fc Tag (MALS verified)

COADS MS
DS
用户评价
货号-规格
价格
Qty.
CD6-M5251-100ug
¥1985.00
CD6-M5251-1mg (500ug X 2)
¥10805.00
合计0件 产品金额¥ 0
新品建议
增项建议

产品详情

  • 分子别名（Synonym）

    CD86,B7-2,B70,CD28LG2,LAB72,MGC34413

  • 表达区间及表达系统（Source）

    Mouse B7-2, Fc Tag (CD6-M5251) is expressed from human 293 cells (HEK293). It contains AA Val 24 - Glu 245 (Accession # NP_062261.3).

    Predicted N-terminus: Val 24

    Request for sequence

  • 蛋白结构（Molecular Characterization）

    B7-2 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 51.9 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 内毒素（Endotoxin）

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度（Purity）

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • 制剂（Formulation）

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储（Storage）

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

推荐产品

数据展示

  • 电泳（SDS-PAGE）

    B7-2 SDS-PAGE

    Mouse B7-2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    B7-2 SEC-MALS

    The purity of Mouse B7-2, Fc Tag (Cat. No. CD6-M5251) is more than 95% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.

    Report

  • 活性（Bioactivity）-ELISA

    B7-2 ELISA

    Immobilized Mouse B7-2, Fc Tag (Cat. No. CD6-M5251) at 5 μg/mL (100 μL/well) can bind Mouse CTLA-4, His Tag (Cat. No. CT4-M52H5) with a linear range of 0.4-13 ng/mL (QC tested).

    Protocol

  • B7-2 ELISA

    Immobilized Biotinylated Mouse CD28, His,Avitag (Cat. No. CD8-M82E3) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Mouse B7-2, Fc Tag (Cat. No. CD6-M5251) with a linear range of 2-39 ng/mL (Routinely tested).

    Protocol

用户评价
发表评论

背景介绍

Cluster of Differentiation 86 (CD86) is also known as B-lymphocyte activation antigen B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily, and is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B72 is expressed at low levels on monocytes and can be upregulated through interferon γ. CD86 is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD86 works in tandem with CD80 to prime T cells. Recent study has revealed that B7-2 promotes the generation of a mature APC repertoire and promotes APC function and survival. Furthermore, the B7 proteins are also involved in innate immune responses by activating NF-κB-signaling pathway in macrophages. CD86 thus is regarded as a promising candidate for immune therapy. CD86+ macrophages in Hodgkin lymphoma patients are an independent marker for potential nonresponse to firstline-therapy.

前沿进展

 
药物研发进展
  • 英文全称：

    Cluster of differentiation 86

  • 中文全称：

    分化群86

  • 种类：

  • 上市药物数量：

    2 详情

  • 临床药物数量：

    5 详情

  • 最高研发阶段：

    批准上市

联系我们
项目合作
查看更多项目
实验案例分析
查看更多案例分析
  • 产品基础信息
  • 产品详情
  • 推荐产品
  • 数据展示
  • 用户评价
  • 背景介绍