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分子别名（Synonym）
AP-TNAP, APTNAP, HOPS, HPPA, HPPC, HPPI, HPPO, TNALP, TNAP, TNS-ALP, TNSALP
表达区间及表达系统（Source）
Cynomolgus ALPL Protein, His Tag (active enzyme) (ALL-C52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 18 - Ser 502 (Accession # G7NUV3).
Predicted N-terminus: Leu 18
Request for sequence蛋白结构（Molecular Characterization）
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 55.4 kDa. The protein migrates as 63-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Cynomolgus ALPL Protein, His Tag (active enzyme) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus ALPL Protein, His Tag (active enzyme) (Cat. No. ALL-C52H3) is more than 90% and the molecular weight of this protein is around 110-140 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Cynomolgus ALPL Protein, His Tag (active enzyme) (Cat. No. ALL-C52H3) at 1 μg/mL (100 μL/well) can bind various dilution ratio of Anti-ALPL Polyclonal Antibody, Rabbit lgG (QC tested).
Protocol
活性（Bioactivity）
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is >40,000 pmol/min/μg (QC tested).
Protocol
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