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分子别名（Synonym）
ADORA2A, adenosine A2a receptor, A2aR, ADORA2, RDC8
表达区间及表达系统（Source）
Human ADORA2A Protein, Flag,His Tag (ADA-H52D3) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Leu 208 & Arg 222 - Ala 412 (Accession # P29274-1).
Predicted N-terminus: Asp
蛋白结构（Molecular Characterization）
This protein carries a flag tag at the N-terminus and a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 61.1 kDa. The protein migrates as 53-58 kDa and 60-120 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumstances should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible with your application, remove glycerol just before the immediate experiment, and NEVER store glycerol-free protein solution.Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
质量管理控制体系（QMS）
- *The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow this link for product information.
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数据展示
电泳（SDS-PAGE）
Human ADORA2A Protein, Flag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human ADORA2A Protein, Flag,His Tag (Cat. No. ADA-H52D3) at 5 μg/mL (100 μL/well) on a Nickel Coated plate (Cat. No. SP-16) can bind Anti-ADORA2A Antibody, Human IgG1 with a linear range of 0.05-1 ng/mL (QC tested).
Protocol
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