Human ADIPOR1 Protein, Flag,His Tag (Detergent)

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Qty.
AD1-H55D3-20ug
¥7350.00
AD1-H55D3-100ug
¥27300.00
AD1-H55D3-500ug
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合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    ACDCR1, CGI-45, CGI45, PAQR1, TESBP1A

  • 表达区间及表达系统(Source)

    Human ADIPOR1 Protein, Flag,His Tag (AD1-H55D3) is expressed from Baculovirus-Insect cells. It contains AA Glu 89 - Leu 375 (Accession # Q96A54).

    Predicted N-terminus: Asp

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    ADIPOR1 Structure

    This protein carries a flag tag at the N-terminus and a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 36.8 kDa.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    This product is not suitable for cell based experiments due to cytotoxicity of DDM.
    DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumstances should you remove DDM and CHS.
    DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
    If glycerol is not compatible with your application, remove glycerol just before the immediate experiment, and NEVER store glycerol-free protein solution.

    Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • 运输(Shipping)

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储(Storage)

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程
  • *The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow the link for product information.

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数据展示

  • 活性(Bioactivity)-ELISA

     ADIPOR1 ELISA

    Immobilized Human ADIPOR1 Protein, Flag,His Tag (Cat. No. AD1-H55D3) at 1 μg/mL (100 μL/well) can bind Anti-ADIPOR1 Antibody, Rabbit IgG1 with a linear range of 0.2-5 ng/mL (QC tested).

    Protocol
  • 活性(Bioactivity)-SPR

     ADIPOR1 SPR

    Human AdipoR1 Antibody captured on Protein G Chip can bind Human ADIPOR1 Protein, Flag, His Tag (Cat. No. AD1-H55D3) with an affinity constant of 84.3 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).

    Protocol

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背景介绍

Adiponectin Receptor 1 (AdipoR1) is a pivotal receptor mediating the metabolic effects of the adipokine adiponectin. AdipoR1 features seven transmembrane domains with an intracellular N-terminus and an extracellular C-terminus responsible for adiponectin binding. AdipoR1 is predominantly expressed in skeletal muscle and can activate adenosine monophosphate-activated protein kinase (AMPK). This activation leads to the phosphorylation of ACC and the subsequent upregulation of CPT-1A, which together critically enhance mitochondrial fatty acid β-oxidation. Dysfunction in this AdipoR1-driven pathway is closely associated with impaired fatty acid oxidation and dyslipidemia. AdipoR1 represents a significant therapeutic target for metabolic disorders involving lipid dysregulation.

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