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产品描述（Product Details）
HRV 3C Protease is highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro and cleaves after the glutamine residue.
应用说明（Application）
Removal of purification tags from fusion proteins.
单位定义（Unit Definition）
One unit will cleave >95% of 100 µg control fusion protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4℃ for 16 h.
纯度（Purity）
>95% as determined by SDS-PAGE.
酶活（Enzyme Activity）
>1 U/μL
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.
Contact us for customized product form or formulation.
存储（Storage）
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state.
- -70°C for 12 months under sterile conditions after reconstitution.
- *该试剂盒配套10× Reaction Buffer中含有DTT，DTT对酶活影响较大，当使用长期贮存或反复冻融的10× Reaction Buffer时，为了保证最大活性，应补加10 mM DTT
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数据展示
电泳（SDS-PAGE）
The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）
>1 Units/µg. One unit will cleave >95% of 100 µg control fusion protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4℃ for 16 h (QC tested).
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