Anti-Doxorubicin Antibody Screening Panel

  • Antibody Internalization Detection Reagent (IGG-PZF2001) monitors endocytosis in live cells
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Qty.
APA-05-40ug
¥2600.00
合计0件 产品金额¥ 0

产品详情

  • 产品描述(Product Details)

    This antibody panel comprises two distinct rabbit monoclonal antibodies against Doxorubicin, with slight differences in their recognition epitopes. Consequently, they may exhibit varying affinity levels towards different Doxorubicin analogs or different conjugates of Doxorubicin and linkers. This panel can be used to conduct preliminary experiments for screening the optimal antibody clone.

  • 组分(Components)

    Cat.No.
    Clone
    Size
    DON-MY2216
    1M2C3
    20 ug
    DON-MY2215
    1M2B1
    20 ug
  • 特异性(Specificity)

    Specifically recognizes Doxorubicin.

  • 抗体来源(Source)

    This panel contains Two anti-Doxorubicin monoclonal antibodies, suitable for pharmacokinetic (PK) detection of ADC with Doxorubicin as the payload. All the anti-Doxorubicin monoclonal antibodies are recombinantly expressed from HEK293, can be used as a capture antibody for detecting conjugated antibodies in ELISA assay.

  • 亚型(Isotype)

    Rabbit IgG | Rabbit Kappa

  • 纯化(Purification)

    Protein A purified / Protein G purified

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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    • 活性(Bioactivity)-ELISA

       Doxorubicin ELISA

      Immobilized Doxorubicin-ADC at 0.1 μg/mL (100 μL/well) can bind Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2C3) (Cat. No. DON-MY2216) with a linear range of 0.12-3.9 ng/mL (QC tested).

      Protocol
    •  Doxorubicin ELISA

      Immobilized Doxorubicin-ADC at 0.1 μg/mL (100 μL/well) can bind Monoclonal Anti-Doxorubicin specific Antibody, Rabbit IgG (1M2B1) (Cat. No. DON-MY2215) with a linear range of 0.12-3.9 ng/mL (QC tested).

      Protocol

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背景介绍

Doxorubicin a cytotoxic anthracycline antibiotic, is an anti-cancer chemotherapy agent. As the payload of ADC drugs, Doxorubicin can act directly on DNA, insert into the double helix strands of DNA, cause the latter to unwind, change the template properties of DNA, and inhibit DNA polymerase, thereby inhibiting the synthesis of both DNA and RNA. Anti-Doxorubicin antibody is a rabbit monoclonal antibody specifically reacts with Doxorubin without other payloads, which is more sensitive than mouse antibody. The anti-Doxorubicin antibody is a useful reagent in PK assay to determine conjugated antibodies.

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