产品详情
产品参数(Product Specifications)
Assay TypeSandwich-ELISAAnalyteVitronectinFormat96T (8×12 strips)ReactivityHumanRegulatory StatusRUOSensitivity<0.375 ng/mLStandard Curve Range0.375 ng/mL-12 ng/mLAssay Time3 hr 20 minSuitable Sample TypeFor the detection and quantitative determination of Vitronectin in stem cell culture supernates, serum, plasma and cell lysates.Sample volume100 μL产品概述(Product Overview)
Vitronectin, a key extracellular matrix glycoprotein, exerts crucial effects on stem cell adhesion, proliferation, differentiation, self-renewal maintenance, cell survival, and integrin-mediated signaling. It exists as a monomeric glycoprotein with a modular structure, containing functional domains that bind to integrins (e.g., αvβ3, αvβ5), heparin, and other matrix components. Different isoforms, including plasma-derived and cell-secreted vitronectin, play distinct roles in stem cell niche regulation, tissue regeneration, and cell-matrix crosstalk—particularly critical for maintaining stem cell pluripotency and guiding directed differentiation.
To support the advancement of stem cell therapy and regenerative medicine research, ACROBiosystems developed Human Vitronectin Quantitative ELISA Kit with strict methodological validation. This kit is specifically designed for the detection and quantitative determination of vitronectin in stem cell culture supernates, serum, plasma, and cell lysates. Additionally, it can be used for the quantitative analysis of Recombinant Vitronectin, premium grade (ACROBiosystems, cat# GMP-VINH19) concentrations—an essential tool for quality control of stem cell culture supplements and evaluation of vitronectin-mediated stem cell behavior.
应用说明(Application)
The kit is developed for the detection and quantitative determination of Vitronectin in cell culture supernates.
It is for research use only.
存储(Storage)
1. Store the unopened kit at 2-8°C upon receipt.
2. Locate the expiration date on the outer packaging and do not use reagents beyond their expiration date.
组分(Materials Provided)
IDComponentsSizeRES103-C01Pre-coated Anti-Vitronectin Antibody Microplate1 plate (8×12 strips)RES103-C02Human Vitronectin Standard10 μg×2RES103-C03Biotin-Anti-Vitronectin Antibody20 μgRES103-C04Streptavidin-HRP50 μLRES103-C0510×Washing Buffer50 mLRES103-C062×Dilution Buffer50 mLRES103-C07Substrate Solution12 mLRES103-C08Stop Solution7 mL质量管理控制体系(QMS)
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数据展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.

For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
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