Human TSLP / IL-7Rα & TSLP R Inhibitor Screening Kit (TR-FRET)

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货号-规格
价格
Qty.
FRT-P012-100tests
¥6300.00
FRT-P012-500tests
¥22050.00
FRT-P012-10000tests (500tests X 20)
¥200000.00
合计0件 产品金额¥ 0

产品详情

  • 优势与特点(FAB)

    1. Simple and fast operation - No complicated washing steps, significantly reducing time.
    2. High batch consistency - Strict control over raw materials and finished product quality, ensuring a stable supply.
    3. Accurate and reliable results - High sensitivity with minimal matrix effects.
    4. High throughput capability - Support multiple product specifications, ideal for high-throughput screening.
    5. Fast completion - Results in just 1 hour.
  • 产品参数(Product Specifications)

    Assay Type
    Competition-TR-FRET
    Format
    100T/500T
    Regulatory Status
    RUO
    Reactivity
    Human
    Assay Time
    1 hr
    Sample volume
    10 μL

  • 产品概述(Product Overview)

    The Human TSLP / IL-7Rα & TSLP R Inhibitor Screening Kit (TR-FRET) is based on a homogeneous (no wash) competition TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer) to screening for inhibitors of human TSLP binding to human IL-7Rα & TSLP R within 0.5-1 hours. It can also be used as a universal detection tool to identify the ability of human TSLP to bind to human IL-7Rα & TSLP R.

  • 存储(Storage)

    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • 组分(Materials Provided)

    ID
    Components
    Size
    FRTP012-C01
    Human TSLP Protein Europium-chelate
    100 tests/500 tests
    FRTP012-C02
    FA Labeled Human IL-7 R alpha & TSLP R
    100 tests/500 tests
    FRTP012-C03
    Anti-TSLP Neutralizing Antibody
    25 μg/100 tests 125 μg/500 tests
    DB-04
    TR-FRET Sample Dilution Buffer, pH7.4
    50 mL/100 tests & 500 tests
    DB-05
    TR-FRET Detection Buffer, pH7.4
    50 mL/100 tests & 500 tests

  • 原理(Assay Principles)

    This Human TSLP / IL-7Rα & TSLP R Inhibitor Screening Kit (TR-FRET) is based on TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer). Use a mixture of biotinylated human TSLP and Europium-chelate labeled streptavidin as the donor and FA labeled human IL-7 R alpha & TSLP R as the acceptor.

    — In the absence of inhibitors for human TSLP binding to human IL-7 R alpha & TSLP R, the donor and acceptor are in close proximity due to the binding of human TSLP and human IL-7 R alpha & TSLP R. Upon excitation with a specific light source, the donor emits a 620 nm signal, which is absorbed by the acceptor, resulting in a 665 nm emission.

    — In the presence of inhibitors that block the binding of human TSLP to human IL-7 R alpha & TSLP R, the donor-acceptor interaction is disrupted, preventing FRET from occurring.

    Assay Principles
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 活性(Bioactivity)-TR-FRET

    Please refer to DS document for the assay protocol.

     TSLP TR-FRET

    In this TR-FRET assay, Human TSLP Protein Europium-chelate is used as the Donor and FA Labeled Human IL-7 R alpha & TSLP R is used as the Acceptor. An inhibition Assay was performed to evaluate the interaction between human TSLP and human IL-7 R alpha & TSLP R using Anti-TSLP Neutralizing Antibody resulting in a typical IC50 of 0.2463 nM (QC tested).

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前沿进展

 
药物研发进展
  • 英文全称:

    Thymic stromal lymphopoietin

  • 中文全称:

    胸腺基质淋巴细胞生成素

  • 种类:

  • 上市药物数量:

    1 详情

  • 临床药物数量:

    23 详情

  • 最高研发阶段:

    批准上市

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