产品详情
产品概述(Product Overview)
The Human Tumor Necrosis Factor Alpha (TNF-α) Detection kit (TR-FRET) can detect the Human Tumor Necrosis Factor Alpha (TNF-α) in homogeneous system within 2 hours, it is highly sensitive, short detection time and easy to use. This kit is specifically designed for the accurate quantitation of human TNF-alpha from cell culture supernatant, serum and plasma.
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
产品参数(Product Specifications)
Assay TypeDetection-TR-FRETAnalyteHuman TNF-αFormat100T/500TReactivityHumanSensitivityLOQ=39 pg/mLAssay Time2 hrSample volume16 μLRange39 pg/mL-2500 pg/mLSample TypeFor human TNF-α detection in human serum, human plasma,cell culture supernatants.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
应用说明(Application)
The kit is developed for quantitative detection of TNF-α in human serum, plasma and cell culture supernates.
It is for research use only.
流程图(Workflow)

存储(Storage)
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table.
质量管理控制体系(QMS)
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数据展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.

Standard curve generated in TR-FRET Sample Dilution Buffer, pH7.4 (DB-04):
稀释线性(Dilution Linearity)
To assess the linearity of the assay, samples spiked with high concentrations of human TNF-α were serially diluted with TR-FRET Sample Dilution Buffer, pH7.4 (DB-04) to produce samples with values within the dynamic range of the assay.

批内差异(Intra-Assay Statistics)
Five Samples of known concentrations were tested repeatedly 16 times in batches to assess within-batch precision and accuracy.

批间差异(Inter-Assay Statistics)
Five Samples of known concentrations were tested repeatedly across three independent analytical batches to evaluate between-batch precision and accuracy.

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