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产品参数（Product Specifications）
Assay TypeFACSAnalyteIL-2, IL-4, IL-5, IL-10, TNF-α, IFN-γFormat50TReactivityHumanRegulatory StatusRUOSensitivityIL-2 (0.25 pg/mL), IL-4 (0.32 pg/mL), IL-5 (1.15 pg/mL), IL-10 (0.1 pg/mL), TNF-α (0.78 pg/mL), IFN-γ (0.29 pg/mL)Standard Curve Range9.8-10000 pg/mL (TNF-α, IFN-γ), 9.8-5000 pg/mL (IL-2, IL-4, IL-5, IL-10)Assay Time3 hrSuitable Sample TypeHuman serum, Human plasma, Cell culture supernatantsSample volume30 μL产品概述（Product Overview）
T helper cells (Th) play a vital role in the adaptive immune system by suppressing or regulating immune responses. After Th actived, it will differentiate into T-helper 1 (Th1) or T-helper 2 (Th2) effector cells. These two subsets are characterized by their distinct cytokine expression pattern and the immune function they mediate. Th1 and Th2 cells each produce a specialized combination of cytokines that depends on type of pathogen that the helper T cell has recognized-some cytokines are more effective than others in the process of eliminating certain invaders. In general, TH1 cytokines play the role of immune killing, while Th2 cytokines play the role of immune protection. Th1/Th2 paradigm provides rationale for developing new vaccines against infectious diseases and novel strategies for the therapy of allergic and autoimmune disorders.
应用说明（Application）
The Human Th1/Th2 Cytokine Kit can be used to quantitatively measure Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-5 (IL-5), Interleukin-10 (IL-10), Tumor Necrosis Factor-α (TNF-α), and Interferon-γ (IFN-γ) protein levels in a single sample.The kit performance has been optimized for analysis of specific cytokines in tissue culture supernatants, EDTA plasma, and serum samples.
It is for research use only.
存储（Storage）
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
组分（Materials Provided）
CatalogProduct NameSizeFMBc007-02Anti-human IL-2 Antibody-coupled APC-beads-D0150 testsIL2-PFS426PE-Labeled Monoclonal Anti-human IL-2 Antibody, Rabbit IgG50 testsFMBc008-02Anti-human IL-4 Antibody-coupled APC-beads-D0550 testsIL4-PFS269PE-Labeled Monoclonal Anti-human IL-4 Antibody, IgG150 testsFMBc014-02Anti-human IL-5 Antibody-coupled APC-beads-C0350 testsFABm030-01PE labeled Monoclonal Anti-Human IL-5 Antibody, Rat IgG150 testsFMBc017-02Anti-human IL-10 Antibody-coupled APC-beads-D0850 testsIL0-PFS428PE labeled Monoclonal Anti-Human IL-10 Antibody, Rat IgG150 testsFMBc010-02Anti-human TNF-α Antibody-coupled APC-beads-D1350 testsTNA-PFM494PE-Labeled Monoclonal Anti-TNF-alpha Antibody, human IgG1 (16H5)50 testsFMBc011-02Anti-human IFN-γ Antibody-coupled APC-beads-D1750 testsIFN-PFM412PE-Labeled Monoclonal Anti-IFN-gamma Antibody, human IgG1 (8C5F8)50 testsFMBf001-02APC Positive Control-010.5 mLFMBf001-01PE Positive Control-010.5 mLFCMP-0296-well Sealing film1 plateFCMP-0196-Well V-Bottom Assay Plate2 piecesFCMB-031* Assay Buffer50 mLFCMB-041*Wash Buffer50 mLIL2-H51P3Calibrator (IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ)5 μg for eachIL5-H52H3Calibrator (IL-5)50 μg原理（Assay Principles）
The Human Th1/Th2 Cytokine Kit-2 is a multiplex bead-based indirect immunofluo-rescence assay, utilizing flow cytometry for data acquisition and classification. The beads conjugated with specific antibody, target protein (IL-2/4/5/10, TNF-α, IFN-γ) in specimens and PE-conjugated detection antibody form a sandwiches complex as bead-analyte-detection antibody. The intensity of PE fluorescence signal in proportion to the target protein in specimens, are assessed by flow cytometry. The present assay has been optimized for quantitatively analysis for the IL-2/4/5/10, TNF-α, IFN-γ in cell culture supernatant, plasma, and serum samples.
质量管理控制体系（QMS）
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数据展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
A double logarithmic fitting curve was plotted for each cytokine( IL-2, IL-4, IL-5, IL-10, TNF-α, IFN-γ). The present curve of IL-2/IL-4/IL-5/IL-10 was ranging from 9.8 pg/ml to 5000 pg/mL, TNF-α/IFN-γ raging from 9.8 pg/mL to 10000 pg/mL .
活性（Bioactivity）-FCM
Please refer to DS document for the assay protocol.
A representative scatter plot showing single bead population in P1 gate (left), the beads conjugated with analyte-sepcific antibody differntiated by APC fluorescence intensity (middle), and fluorescence signal in propotion to concentration of the analyte was measured for each cytokine in PE channel (right). Data was generated on Beckman Cytoflex S Flow Cytometer, equipped with blue laser (561 nm) and red laser (640 nm).
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